Insect Cells-Baculovirus System for the Production of Difficult to Express Proteins: From Expression Screening for Soluble Constructs to Protein Quality Control

February 5, 2022 0 Comments

Rapid preparation of proteins for functional and structural analysis is a major challenge both in academia and industry. The number potential targets continuously increases and many are difficult to express proteins which, when produced in bacteria, result in insoluble and/or misfolded recombinant proteins, protein aggregates, or unusable low protein yield. We focus here on the baculovirus expression vector system …

Defining ELISA Detection Options

October 8, 2020 0 Comments

Defining ELISA Detection Choices In performing an ELISA, enzymes are employed as a label that can produce the power to detect a sign that signifies an antibody has certain to a particular antigen. The method can use varied several types of enzymes and enzyme substrates, with barely differing strategies of incorporating them. Relying on the …