Anti-CD25 monoclonal antibody enhances the protective efficacy of Schistosoma japonicum GST vaccine via inhibition of CD4+CD25+Foxp3+ regulatory T cells

February 15, 2022 0 Comments

The effect of anti-CD25 monoclonal antibody (anti-CD25 mAb) on the protection efficacy of Schistosoma japonicum 26 kDa GST (glutathione-S-transferase) vaccine was evaluated. Mice were immunized with GST before infection with S. japonicum cercariae and then injected with anti-CD25 mAb. The worm reduction rate was promoted from 24.18% in mice with GST immunization to 47.09% in …

Adaptation of an ELISA assay for detection of IgG 2a responses against therapeutic monoclonal antibodies in a mouse immunization model

February 15, 2022 0 Comments

Biotherapeutic monoclonal antibodies (mAb) play important roles in clinical medicine but their potential to elicit immune responses in patients remains a major issue. In a study designed to investigate the effect of aggregation on immunogenic responses, mice were immunized with two monoclonal antibodies (mAb1 and mAb2). Serum levels of total IgG, IgG1, and IgG2a were measured …

Commercially Obtainable ELISA Kits for Measuring Adalimumab and Anti-adalimumab

October 8, 2020 0 Comments

A Comparative Analysis of 4 Commercially Obtainable ELISA Kits for Measuring Adalimumab and Anti-adalimumab Antibodies   Background: Therapeutic drug monitoring of tumor necrosis issue inhibitors, resembling adalimumab, is more and more being carried out for the administration of autoimmune ailments. Nevertheless, there might be vital variation in drug and antibody concentrations obtained by totally different assay strategies. The intention …