Adaptation of an ELISA assay for detection of IgG 2a responses against therapeutic monoclonal antibodies in a mouse immunization model
Biotherapeutic monoclonal antibodies (mAb) play important roles in clinical medicine but their potential to elicit immune responses in patients remains a major issue. In a study designed to investigate the effect of aggregation on immunogenic responses, mice were immunized with two monoclonal antibodies (mAb1 and mAb2). Serum levels of total IgG, IgG1, and IgG2a were measured by ELISA. An anti-mouse IgG2a monoclonal detection antibody cross-reacted with mAb2 but not mAb1, leading to high background when the ELISA plate was coated with mAb2.
The problem was solved by use of a goat anti-mouse IgG2a polyclonal antibody that demonstrated the required specificity. IgG2a responses were similar for monomer- or aggregate-coated ELISA plates. The results demonstrate the importance of assessment of the specificity of individual reagents when measuring antibody responses against therapeutic antibodies by ELISA.
Regression of Triple-Negative Breast Cancer in a Patient-Derived Xenograft Mouse Model by Monoclonal Antibodies against IL-12 p40 Monomer
Generation, Characterization and Protective Ability of Mouse Monoclonal Antibodies Against the HA of A (H1N1) Influenza Virus
Production and application of mouse monoclonal antibodies targeting linear epitopes in pB602L of African swine fever virus
- A total of eight mouse mAbs were obtained, and their binding epitopes were screened by Western blot using an overlapping set of polypeptides from pB602L. Three linear epitopes were identified and designated epitope 1 (366ANRERYNY373), epitope 2 (415GPDAPGLSI423), and epitope 3 (498EMLNVPDD505).
- Based on the epitope recognized, the eight mAbs were placed into three groups: group 1 (B2A1, B2F1, and B2D10), group 2 (B2H10, B2B2, B2D8, and B2A3), and group 3 (B2E12).
- The mAbs B2A1, B2H10, and B2E12, each representing one of the groups, were used to detect pB602L in ASFV-infected porcine alveolar macrophages (PAMs) and pig tissues, using an indirect fluorescence assay (IFA) and immunohistochemical staining, respectively.
- The results showed that pB602L was detectable with all three mAbs in immunohistochemical staining, but only B2H10 was suitable for detecting the proteins in ASFV-infected PAMs by IFA.
- In summary, we developed eight anti-pB602L mouse mAbs recognizing three linear epitopes in the protein, which can be used as reagents for basic and applied research on ASFV.
Anti-IAPP Monoclonal Antibody Improves Clinical Symptoms in a Mouse Model of Type 2 Diabetes
Mouse Monoclonal Antibody |
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28297-100Tests | SAB | 100 Tests | 494.4 EUR |
Mouse Monoclonal Antibody |
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28297-25Tests | SAB | 25 Tests | 250.8 EUR |
Mouse Monoclonal Antibody |
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28298-100Tests | SAB | 100 Tests | 598.8 EUR |
Mouse Monoclonal Antibody |
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28298-25Tests | SAB | 25 Tests | 294 EUR |
Mouse Monoclonal antibody AntiCCND2 |
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CCND2ACMO | ImmunoStep | 0,1 mg | 418.8 EUR |
PCNA Mouse Monoclonal Antibody |
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37978-100ul | SAB | 100ul | 302.4 EUR |
PCNA Mouse Monoclonal Antibody |
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37978-50ul | SAB | 50ul | 224.4 EUR |
EGFR Mouse Monoclonal Antibody |
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37996-100ul | SAB | 100ul | 302.4 EUR |
EGFR Mouse Monoclonal Antibody |
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37996-50ul | SAB | 50ul | 224.4 EUR |
CD20 Mouse Monoclonal Antibody |
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38003-100ul | SAB | 100ul | 302.4 EUR |
CD20 Mouse Monoclonal Antibody |
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38003-50ul | SAB | 50ul | 224.4 EUR |
CD23 Mouse Monoclonal Antibody |
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38004-100ul | SAB | 100ul | 302.4 EUR |
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38004-50ul | SAB | 50ul | 224.4 EUR |
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38005-100ul | SAB | 100ul | 302.4 EUR |
CD68 Mouse Monoclonal Antibody |
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38005-50ul | SAB | 50ul | 224.4 EUR |
CK19 Mouse Monoclonal Antibody |
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38006-100ul | SAB | 100ul | 302.4 EUR |
CK19 Mouse Monoclonal Antibody |
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38006-50ul | SAB | 50ul | 224.4 EUR |
p53 Mouse Monoclonal Antibody |
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38007-100ul | SAB | 100ul | 302.4 EUR |
p53 Mouse Monoclonal Antibody |
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38007-50ul | SAB | 50ul | 224.4 EUR |
CK7 Mouse Monoclonal Antibody |
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38009-100ul | SAB | 100ul | 302.4 EUR |
CK7 Mouse Monoclonal Antibody |
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38009-50ul | SAB | 50ul | 224.4 EUR |
CK8 Mouse Monoclonal Antibody |
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38010-100ul | SAB | 100ul | 302.4 EUR |
CK8 Mouse Monoclonal Antibody |
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38010-50ul | SAB | 50ul | 224.4 EUR |
CK16 Mouse Monoclonal Antibody |
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38011-100ul | SAB | 100ul | 302.4 EUR |
CK16 Mouse Monoclonal Antibody |
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38011-50ul | SAB | 50ul | 224.4 EUR |
CDX2 Mouse Monoclonal Antibody |
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38012-100ul | SAB | 100ul | 302.4 EUR |
CDX2 Mouse Monoclonal Antibody |
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38012-50ul | SAB | 50ul | 224.4 EUR |
GFAP Mouse Monoclonal Antibody |
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38014-100ul | SAB | 100ul | 302.4 EUR |