HRP-conjugated Anti-EPO antibodies for direct rhEPO detection – proof of concept

February 15, 2022 0 Comments

Anti-doping testing for recombinant human erythropoietin (EPO) is routinely performed by gel electrophoresis followed by western blot analysis with primary and secondary antibodies. The two antibody steps add more than 24 hours to the testing time of a purified sample. The aim of this study was to test the concept of using directly horseradish-peroxidase (HRP)-conjugated …

Secondary immunoglobulin responses of BALB/c mice previously stimulated with goat anti-mouse IgD.

February 15, 2022 0 Comments

Intravenous injection of goat antibodies to mouse IgD (GAMD) into BALB/c mice has been shown to induce vigorous T-cell dependent immunoglobulin responses, particularly of the IgG1 and IgE isotypes. We have confirmed these findings and show that IgA responses are also triggered in this model. Since the study of IgE regulation in allergic individuals is …

An Anti-HER2 Monoclonal Antibody H 2 Mab-41 Exerts Antitumor Activities in Mouse Xenograft Model Using Dog HER2-Overexpressed Cells

February 15, 2022 0 Comments

Overexpression of human epidermal growth factor receptor 2 (HER2) has been reported in a variety of cancer types, including breast, lung, gastric, pancreatic, and colorectal cancers. Trastuzumab, a humanized anti-HER2 monoclonal antibody (mAb), has been shown to provide significant survival benefits in HER2-overexpressing breast cancer and gastric cancer patients. Previously, an anti-HER2 mAb, H2Mab-41 (IgG2b, …

Adaptation of an ELISA assay for detection of IgG 2a responses against therapeutic monoclonal antibodies in a mouse immunization model

February 15, 2022 0 Comments

Biotherapeutic monoclonal antibodies (mAb) play important roles in clinical medicine but their potential to elicit immune responses in patients remains a major issue. In a study designed to investigate the effect of aggregation on immunogenic responses, mice were immunized with two monoclonal antibodies (mAb1 and mAb2). Serum levels of total IgG, IgG1, and IgG2a were measured …

In-House Developed ELISA Indicates High Prevalence of Anti- Echinococcus granulosus IgG in Sheep Population-An Update from Pakistan

February 15, 2022 0 Comments

Cystic echinococcosis (CE) is a World Health Organization (WHO)-listed neglected tropical farm economy jeopardizing and public health concern disease. This study was aimed at furnishing sero-epidemiological baseline data of CE in sheep in Pakistan, where data are non-existent. For this purpose, two sheep-rich provinces of Pakistan were selected, and 728 sheep sera were collected using …

Preparation of rabbit polyclonal antibody against porcine gasdermin D protein and determination of the expression of gasdermin D in cultured cells and porcine tissues

February 15, 2022 0 Comments

Gasdermin-D (GSDMD) is a member of the gasdermin (Gsdm) protein family, and its cleavage by inflammatory cysteine proteases (caspases, CASPs) is a critical event in cell pyroptosis. The role and functions of GSDMD on mice and humans are widely studied, but its expression, structure, and function in other species are less known. In the present …

Insect Cells-Baculovirus System for the Production of Difficult to Express Proteins: From Expression Screening for Soluble Constructs to Protein Quality Control

February 5, 2022 0 Comments

Rapid preparation of proteins for functional and structural analysis is a major challenge both in academia and industry. The number potential targets continuously increases and many are difficult to express proteins which, when produced in bacteria, result in insoluble and/or misfolded recombinant proteins, protein aggregates, or unusable low protein yield. We focus here on the baculovirus expression vector system …

Commercially Obtainable ELISA Kits for Measuring Adalimumab and Anti-adalimumab

populationhealthsciences
October 8, 2020 0 Comments

A Comparative Analysis of 4 Commercially Obtainable ELISA Kits for Measuring Adalimumab and Anti-adalimumab Antibodies   Background: Therapeutic drug monitoring of tumor necrosis issue inhibitors, resembling adalimumab, is more and more being carried out for the administration of autoimmune ailments. Nevertheless, there might be vital variation in drug and antibody concentrations obtained by totally different assay strategies. The intention …

ELISA Kits to Confirm the Absence of Transmission in Malaria

populationhealthsciences
October 8, 2020 0 Comments

Info On ELISA And ELISA Sensitivity ELISA is actually a diagnostic instrument that’s usually utilized in plant pathology and medication. It is usually utilized in numerous industries as a instrument for high quality management. It includes the detection of the precise substance (or analyte) being analyzed quantitatively or qualitatively in a liquid pattern. This method …

Defining ELISA Detection Options

populationhealthsciences
October 8, 2020 0 Comments

Defining ELISA Detection Choices In performing an ELISA, enzymes are employed as a label that can produce the power to detect a sign that signifies an antibody has certain to a particular antigen. The method can use varied several types of enzymes and enzyme substrates, with barely differing strategies of incorporating them. Relying on the …